Cartilage- sparing properties of Equine Omega Complete in an organ culture model of cartilage inflammation- Abstract
The purpose of this study was to determine anti-inflammatory and/or chondroprotective effects of Equine Omega Complete (EOC) on cartilage explants stimulated with lipopolysaccharide (LPS). Explants were aseptically prepared from the intercarpal joints of 17 market-weight pigs and placed in culture at 37°C for a total of 120 hours. For the final 96 hours, explants were conditioned with a simulated digestion extract of EOC (0, 36 or 180 μL/mL), and for the final 48 hours explants were stimulated with LPS (0 or 15µg/mL). Media was removed and replaced every 24 hours. Samples from the final 48 hours were analyzed for biomarkers of cartilage inflammation [prostaglandin E2 (PGE2) and nitric oxide (NO)] and cartilage structure [glycosaminoglycan (GAG)]. At the end of the culture period cartilage explants were stained for an estimate of cell viability. Stimulation of unconditioned explants with LPS significantly increased media concentrations of PGE2, GAG and NO compared with that from unstimulated explants. LPS stimulation did not significantly affect cell viability. Both concentrations of EOC prevented significant LPS-stimulated cartilage release of GAG without impairing chondrocyte viability. No other effects of treatment were observed. These data provide evidence for a non-cytotoxic, chondroprotective effect of EOC in cartilage. This in vitro experiment supports the use of EOC in protecting against the detrimental effects of inflammation on cartilage structure.
Keywords: arthritis; cartilage; equine; nutraceuticals; polyunsaturated fatty acids.
Copyright © 2022. Published by Elsevier Inc.
Based on Equine Science
Equine Omega Complete is an all in one prevention supplement for horses. Targeting joint, gut, respiratory, reproduction muscle and skin and coat health in horse, the supplement has received proven results in a recent joint study at the University of Guelph, Department of Animal Biosciences.
Southern Equine Distributing announces research results from their recent trial at the University of Guelph. The study entitled “Cartilage-sparing properties of Equine Omega Complete (EOC) in an organ culture model of cartilage inflammation” was conducted by Dr. Wendy Pearson, PhD student Anna Garland, and undergraduate student Corina Weirenga, in the Department of Animal Biosciences. The study was designed to determine the ability of EOC to protect cartilage from the damaging effects of inflammation. The researchers pre-treated live cartilage pieces with EOC, while some were left untreated, and then all the cultures were stimulated to ‘behave’ arthritic. In cartilage pieces that were not pre-treated with EOC, the stimulus caused breakdown of cartilage structure, which was measured by loss of an important building block of cartilage (called ‘GAG’) from the cartilage pieces. This loss of cartilage building blocks was prevented by pre-treating the cartilage with EOC, at a dose approximating 60 and 180mL per day for a 500kg horse. Furthermore, exposing cartilage to EOC at these doses was completely safe for the cells within the cartilage pieces.
The study provides evidence for the ability of EOC to protect cartilage structure of horses at risk for joint inflammation and/or arthritis, and supports use of the product to preserve health of joints.
Figure 1: GAG loss from cartilage between 0 and 48 h of culture with an inflammatory stimulus. Loss of GAG indicates damage to cartilage structure; this loss was lower when cartilage was exposed to EOC at 1 and 3 times the manufacturer recommended dose.
Figure 2: Small discs of cartilage are removed from the joint surface (A) and placed into a nutrient broth to keep the cartilage pieces alive (B).
Figure 3: GAG analysis is conducted by placing a small volume of the nutrient broth pictured in 2B above and applying a blue stain which attaches to the GAG molecules present in the broth. When the stain attaches, the blue colour changes to pink – the more GAG present, the more pink the sample looks. An instrument called a spectrophotometer reads the change in colour, and the absorbance from the spectrophotometer is converted to a concentration of GAG in the sample.