#EOC Findings Based On Science

Equine Omega Complete is an all in one prevention supplement for horses. Targeting joint, gut, respiratory, reproduction muscle and skin and coat health in horse, the supplement has received proven results in a recent joint study at the University of Guelph, Department of Animal Biosciences.

Southern Equine Distributing announces research results from their recent trial at the University of Guelph. The study entitled “Cartilage-sparing properties of Equine Omega Complete (EOC) in an organ culture model of cartilage inflammation” was conducted by Dr. Wendy Pearson, PhD student Anna Garland, and undergraduate student Corina Weirenga, in the Department of Animal Biosciences. The study was designed to determine the ability of EOC to protect cartilage from the damaging effects of inflammation. The researchers pre-treated live cartilage pieces with EOC, while some were left untreated, and then all the cultures were stimulated to ‘behave’ arthritic. In cartilage pieces that were not pre-treated with EOC, the stimulus caused breakdown of cartilage structure, which was measured by loss of an important building block of cartilage (called ‘GAG’) from the cartilage pieces. This loss of cartilage building blocks was prevented by pre-treating the cartilage with EOC, at a dose approximating 60 and 180mL per day for a 500kg horse. Furthermore, exposing cartilage to EOC at these doses was completely safe for the cells within the cartilage pieces. 

 The study provides evidence for the ability of EOC to protect cartilage structure of horses at risk for joint inflammation and/or arthritis, and supports use of the product to preserve health of joints. 


Figure 1: GAG loss from cartilage between 0 and 48 h of culture with an inflammatory stimulus. Loss of GAG indicates damage to cartilage structure; this loss was lower when cartilage was exposed to EOC at 1 and 3 times the manufacturer recommended dose.


Figure 2: Small discs of cartilage are removed from the joint surface (A) and placed into a nutrient broth to keep the cartilage pieces alive (B).

Figure 3: GAG analysis is conducted by placing a small volume of the nutrient broth pictured in 2B above and applying a blue stain which attaches to the GAG molecules present in the broth. When the stain attaches, the blue colour changes to pink – the more GAG present, the more pink the sample looks. An instrument called a spectrophotometer reads the change in colour, and the absorbance from the spectrophotometer is converted to a concentration of GAG in the sample.